Journal: Cells

Article Title: Multiplexed Digital PCR Reference Gene Measurement for Genomic and Cell-Free DNA Analysis

doi: 10.3390/cells14191544

Figure Lengend Snippet: Reference gene measurement and HER2 ratio analysis of breast carcinoma cell line-derived hgDNA. ( A ) Reference gene panel was tested using HER2+ gDNA and its matched lymphoblast gDNA (HER2− gDNA). Error bar for the individual genes show range. The error bar for the average shows the expanded measurement uncertainty.; ( B ) HER2 multiplex panel was tested using HER2+ gDNA and its matched lymphoblast gDNA (HER2−). Symbols in black represent HER2+ gDNA, and symbols in grey represent HER2− gDNA. Circle, square, triangle, diamond, hexagon, and down-pointing triangle represent HBB, RPPH1, RPS27A, DCK, PMM1 and the average respectively. Each symbol shows individual measurements.

Article Snippet: Commercially available human genomic DNA (gDNA) isolated from the HCC1954 breast carcinoma cell line with HER2 copy number gain (ATCC CRL-2338D) and paired healthy control gDNA (ATCC CRL-2339D) isolated from the HCC1954 BL EBV-transformed lymphoblastoid cell line (both ATCC, Manassas, VA, USA) were also digested prior to dPCR application as described above.

Techniques: Derivative Assay, Multiplex Assay

Journal: Journal of the American Chemical Society

Article Title: Encapsulation of Gold-Based Anticancer Agents in Protease-Degradable Peptide Nanofilaments Enhances Their Potency

doi: 10.1021/jacs.2c09820

Figure Lengend Snippet: Cell Viability of gold anticancer agents, gold-loaded peptide nanofilaments ( PD+1 , PD+2 , AD+1 , AD+2 ), and peptides ( PD , AD ) in three human cell lines. Caki-1 (renal cancer), MDA-MB-231 (Triple Negative Breast Cancer), and IMR-90 (lung fibroblasts) incubated with drug-loaded peptide for 72 h. A) Compound 1 , B) Compound 2 . (* represents P <0.05, ** represents P <0.01, *** represents P <0.001).

Article Snippet: Human breast adenocarcinoma line MDA-MB-231 and human lung fibroblast line IMR-90 were obtained from the American Type Culture Collection (ATCC; Manassas, Virginia, USA) and cultured with Dulbecco’s modified Eagle’s medium (DMEM; Fisher Scientific) containing 10 % fetal bovine serum, certified, heat inactivated, US origin (FBS; Fisher Scientific), 1 % minimum essential media (MEM) nonessential amino acids (NEAA; Fisher Scientific), and 1 % penicillin–streptomycin (PenStrep; Fisher Scientific).

Techniques: Incubation

Journal: CNS Neuroscience & Therapeutics

Article Title: GPR30‐mediated estrogenic regulation of actin polymerization and spatial memory involves SRC‐1 and PI3K‐mTORC2 in the hippocampus of female mice

doi: 10.1111/cns.13108

Figure Lengend Snippet: Selective activation of mTORC2 reversed G15‐induced spatial memory disorder and actin depolymerization. The sham animals were in diestrous cycling (evidenced by vaginal smear). The mice injected with DMSO were used as the control. A, Flowchart of the experiments. B, A4 reversed G15‐induced learning impairment from days 3 to day 5. C, The swimming tracks of mice in each quadrant. D, The time spent in the target quadrant of animals. E‐G, The total distance traveled and swimming speed. H, A4 rescued G15‐induced downregulation of p‐AKT. I, A4 reversed G15‐induced decrease in F‐actin/G‐actin ratio. A4: mTORC2 activator A‐443654. Data are shown as the mean ± SEM. ** P < 0.01 compared with other groups (repeated measures of two‐way ANOVA, LSD test for water maze test and one‐way ANOVA, LSD test for Western blot analysis)

Article Snippet: The mTORC2‐specific activator A4 (HY‐10425; Medchem Express, Shanghai, China) was prepared with DMSO and intraperitoneally injected with a dose of 2.5 mg/kg body weight as previous reports., Animals in the DMSO and/or sham groups received an injection of an equal amount of vehicle (60% DMSO + 40% sterile saline solution) as indicated in each experiment.

Techniques: Activation Assay, Injection, Control, Western Blot